Journal: Investigative Ophthalmology & Visual Science

Article Title: A Novel Murine Model for Lupus-Like Ocular Chronic Graft-Versus-Host Disease

doi: 10.1167/iovs.65.6.20

Figure Lengend Snippet: Histological changes of the cornea in the lupus-like cGVHD model. The cornea underwent histological analysis at week 2, week 6, and week 10 following transplantation ( n = 5 mice). ( A ) Immunofluorescence staining of corneal fiber nerves stained with β-III Tubulin in corneal whole mounts (Bar = 500 µm) and higher-magnification images of the central cornea (Bar = 100 µm). ( B ) H&E staining was performed to assess the histological changes in the central cornea (Bar = 50 µm). ( C ) Higher-magnification images of the central cornea were obtained to quantitatively assess the number of corneal endothelial cells ( blue arrows ; Bar = 25 µm). ( D ) Quantification of corneal endothelial cell density in two groups. Data were presented as mean ± SD. ns = not significant. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: After blocking with PBS containing 5% bovine serum albumin (BSA) and 0.3% triton (Sigma), the corneas were incubated with neuron-specific marker β-III tubulin antibody (1:50; ab52623; Abcam, Waltham, MA, USA) at 4°C overnight.

Techniques: Transplantation Assay, Immunofluorescence, Staining

Journal: Molecular and cellular neurosciences

Article Title: Expression of the Human Herpesvirus 6A latency-associated transcript U94A impairs cytoskeletal functions in human neural cells

doi: 10.1016/j.mcn.2022.103770

Figure Lengend Snippet: hOPCs infected with GFP+ control and U94A+ lentiviral vectors were exposed to specific growth factors to induce oligodendrocyte maturation, and immunofluorescent analysis was conducted to compare efficiency of differentiation. Processes were visualized by (A) immunofluorescence against β tubulin and (B) digitally reconstructed and analyzed using Imaris FilamentTracer. Quantification of process (C) length, (D) area, and (E) branching complexity were were compared between U94A+ cells and controls. The graphs are displayed as mean ± SEM of n=3 independent biological samples. *p <0.05 by paired two-tailed t-test. No significant difference between U94A and control in process length or area.

Article Snippet: Seven days post-infection, cells were fixed and immunostained for the neuron-specific cytoskeletal marker β III tubulin to visualize processes, and images were reconstructed and analyzed using Imaris FilamentTracer ( , ).

Techniques: Infection, Control, Immunofluorescence, Two Tailed Test

Journal: Molecular and cellular neurosciences

Article Title: Expression of the Human Herpesvirus 6A latency-associated transcript U94A impairs cytoskeletal functions in human neural cells

doi: 10.1016/j.mcn.2022.103770

Figure Lengend Snippet: hCXNs were infected at five days in vitro with GFP control and U94A/GFP lentiviral vectors, and (A) immunofluorescence analysis for βIII tubulin was conducted at seven days post-infection. (B) Imaris FilamentTracer analysis quantification of (C) neurite length, (D) neurite area, and (E) Sholl analysis of neurite complexity was compared between U94A+ cells and GFP controls. The graphs are displayed as mean ± SEM of n≥3 independent biological samples; ****p<0.0001 U94A vs. control, two-way ANOVA; ****p < 0.0001, Holm-Sidak multiple comparison test. No significant difference between U94A and control in neurite area and neurite length by paired, two-tailed t-test.

Article Snippet: Seven days post-infection, cells were fixed and immunostained for the neuron-specific cytoskeletal marker β III tubulin to visualize processes, and images were reconstructed and analyzed using Imaris FilamentTracer ( , ).

Techniques: Infection, In Vitro, Control, Immunofluorescence, Comparison, Two Tailed Test